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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant job in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really nonetheless out there.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.

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This tutorial outlines even more probable optimization of cellular and immunoassay parameters to make sure the very best benefits are...

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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to make certain the very best results are...

Antibodies bound to the blocking peptide no longer bind for the epitope about the goal protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.

Target Information Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development more info via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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